In field where flow cytometers are used, the following problems occur with conventional method using tube with filter.
□Filtration is a pain
□It’s a lot of work and takes a lot of time
□Total cost is high
To solve such problems, we developed and sell this “Centrifugal filtration plate” and “96-well compatible microplate mesh seal“!
✓Can process multiple samples efficiently
✓Prevent contamination per well
✓Unit cost of experiment (filtration cost) can be reduced
Use high homogeneous mesh pores of 30, 70 and 100 μm.
① Use stacked on your microplate
② Sample is dispensed into each well with using multichannel pipette or automatic dispenser
③ Filtration with centrifuge *Recommended: 300g x 30 seconds
Before cell filtration
After cell filtration
Set a centrifugal filtration plate on a conventional 96-well round bottom plate and pass cells through filter → centrifugation
Evaluation point: Presence or absence of contamination after plate centrifugation
Presence or absence of contamination
HEK293T not expressing GFP and HEK293T expressing GFP (human fetal kidney epithelial cell) are filtered in adjacent wells and judged by flow cytometry
① Cells were stripped with trypsin/EDTA and centrifuged.
② Cells were washed with PBS.
③ Cells were diluted with PBS to make about 2 x 104 cells/200 μL.
④ PI was added to make a final concentration of 0.25 μg/mL.
⑤ A centrifugal filtration plate was set on a 96-well plate.
⑥ Cells were added to wells in 200 μL.
⑦ The plate was centrifuged at 300 g x 30 s.
⑧ It was measured with flow cytometer (Guava easyCyte 8HT) .
*Measurement was made only within the red frame.
It is being put in a centrifugal filtration plate
There was no suspension left in the filter.
After centrifugation
・After centrifugation, there was no contamination in another well.
・This time, it was centrifuged at 300 g x 30 seconds, and all the suspension had fallen to the bottom.
| Product name | CSCRIE Centrifugal Filtration Plate |
|---|---|
| Standard | Compatible with 96 and 384-well plate |
| Materials | Plate : polystyrene Mesh : polyester fiber Adhesive : silicone |
