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CS IHC-Fencing Seal

Water-repellent seal surrounding specimen to prevent reagent outflow on

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Overview

Immunohistochemistry (IHC) is a very common technique to visualize target antigens on the tissue specimen mounted on the glass slide by using antibodies. Not only general IHC, but this technique is still used in most advanced technique, such as imaging mass cytometry.

In the step of incubation with antibody solution in IHC, researchers have to draw a circle/square around the tissue specimen on the glass before applying solution to slide. Common method to draw a line is by using a special water repellent barrier pen (e.g. PAP pen). This step is essential for confining a low volume of antibody solution in the defined area efficiently.

However, insufficient drawing such as a gap in the line, too narrow or thin line, or applying antibody solution before the line completely dries up are common reasons of the leak of antibody solution. And that may cause incomplete incubation of antibody and result in the staining error. Occasionally, two (or more ) different sections mounted on the same slide have to be stained by using different antibodies. When to do this, leaking and mixing of different antibodies or reagents will lead incorrect data (or no data). Therefore, alternative ways have been anticipated among many researchers to avoid all troubles arise from hydrophobic pens.

We have a good solution for you –

Our IHC-fencing seal, is a water repellent barrier seal for immunohistochemistry staining procedure, replaces conventional hydrophobic barrier pen.

Just take the seal from mounting and put it on the slide. This simple procedure will minimize all troubles caused by hydrophobic barrier pens.

CS IHC-Fencing Seal Features

・Stable : This seal is resistant to general buffers, chemicals and detergent (e.g. Triton X-100), and high temperature (70℃.)
・No leak : As there is no gap, solution will be well-retained in the circle or square.*
 * Please remove air bubbles under the frame and attach firmly.
・Saving your time : No need to wait until the line dries like hydrophobic pens. Just put it and go!
・Easy to remove : Small tab supports fast and easy removal.
・No overflow : You are free from a common trouble of hydrophobic barrier pens.
・Earth Friendly : No organic solvent is used unlike conventional hydrophobic barrier pen.
・Variety : Available in two types (Circle or Square) with different sizes.

Performance evaluation at a joint research institution

Evaluation points

1. Adherence property throughout the immunohistochemistry staining procedure
2. No outflow
3. Usability (Easy to remove and attach)

Experimental conditions

PBST: 0.1% Triton X 100 containing phosphate buffered saline
PBSMT Composition: 0.1% Triton X 100 and 2% skim milk containing phosphate buffered saline 

Evaluated products

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type A

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type B

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type C

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type D

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type E

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type F

Procedure

1. Pinch a tab and remove the CS IHC seal from mounting.

2. Put the CS IHC seal onto the glass slides.

3. Immerse the glass slides in the staining jar with 150 mL of PBSMT and incubate them at room temperature for 1 hour.

4. Remove the glass slides from the staining jar and place them in the moisture chamber.

5. Apply primary antibody solution (diluted in PBSMT) to the glass slide.

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type A
Recommended antibody
solution volume : 200 μL

Minimum antibody
solution volume : 150 μL

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type B
Recommended antibody
solution volume : 80 μL

Minimum antibody
solution volume : 50 μL

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type C
Recommended antibody
solution volume : 100 μL

Minimum antibody
solution volume : 60 μL

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type D
Recommended antibody
solution volume : 10 μL

Minimum antibody
solution volume : 5 μL

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type E
Recommended antibody
solution volume : 40 μL

Minimum antibody
solution volume : 20 μL

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type F
Recommended antibody
solution volume : 60 μL

Minimum antibody
solution volume : 40 μL

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

6. Incubate slides overnight at 4 deg C.

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

7. Rinse the slides three times for 5 minutes of each with PBST.

8. Remove slides from the staining jar, and remove any excess moisture from the slides.

9. Place them it in the moisture chamber, and apply 150 μL of secondary antibody solution (diluted in PBSMT) to the glass slide. Incubate them at room temperature for 30 minutes.

10. Rinse the slides three times for 5 minutes of each, and wipe away any excess wash buffer.

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,
water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Type A-F from the top to bottom.

Summary

Under the general IHC protocol, IHC seal completely retained throughout the procedure.
・Antibody solution tightly kept within the seal of circle/square. No outflow with recommended volume.
・The handle tab makes it easy to remove from mounting.

Product details・Online order

water repellent barrier pen, IHC, immunohistochemistry, glass slide, alternative, PAP pen, IHC-seal,

Product name CS IHC-Fencing Seal
Applications Compatible with microscope glass slide
6types(A、B、C、D、E、F)
Materials

PET(low adhesion tape)

Size
(mm)

Type A:Outer diameter  55x 25(Inner diameter:47x 17)
Type B:Outer diameter  25x 25(Inner diameter:17x 17)
Type C:Outer diameter  55x 25(Inner diameter:21.5x 17)
Type D:Outer diameter φ11(Inner diameter:φ5)
Type E:Outer diameter φ16(Inner diameter:φ10)
Type F:Outer diameter φ21(Inner diameter:φ15)
Common:Thickness 0.155

Type A

Product Code Description Q’ty Price
R-CSC091-02 CS IHC-Fencing Seal Type A 30 pieces Contact us
R-CSC091-01 CS IHC-Fencing Seal Type A 180 pieces

 

Type B

Product Code Description Q’ty Price
R-CSC092-02 CS IHC-Fencing Seal Type B 30 pieces Contact us
R-CSC092-01 CS IHC-Fencing Seal Type B 270 pieces

 

Type C

Product Code Description Q’ty Price
R-CSC093-02 CS IHC-Fencing Seal Type C 30 pieces Contact us
R-CSC093-01 CS IHC-Fencing Seal Type C 180 pieces

 

Type D

Product Code Description Q’ty Price
R-CSC094-02 CS IHC-Fencing Seal Type D 30 pieces Contact us
R-CSC094-01 CS IHC-Fencing Seal Type D 270 pieces

 

Type E

Product Code Description Q’ty Price
R-CSC095-02 CS IHC-Fencing Seal Type E 30 pieces Contact us
R-CSC095-01 CS IHC-Fencing Seal Type E 270 pieces

 

Type F

Product Code Description Q’ty Price
R-CSC096-02 CS IHC-Fencing Seal Type F 30 pieces Contact us
R-CSC096-01 CS IHC-Fencing Seal Type F 270 pieces

 

Chemical Resistance Chart

Chemicals Condition Resistance
0.1% TritonX-100 Yes
Methanol -20 C, 20min Yes
Acetone -20 C, 20min Yes
4% PFA RT, 20min Yes
1% SDS RT, 20min Yes
50% DMSO RT, 20min Yes
50% DMF RT, 1h Yes
Xylene substitute RT, 3 x 5min Yes
Xylene RT, 3 x 5min OK but sometimes detach itself
100% Ethanol RT, 3 x 5min Yes
70% Ethanol RT, 3 x 5min Yes
Antigen retrieval by water bath 100C, 1h Yes
Antigen retrieval by autoclave 121C, 20 min Yes, but Xylene used slide does not work.

CONTACT

0774-29-9037

Weekday 8:30am - 5:30pm
as Japan time