CS IHC-Fencing Seal

Immunohistochemistry (IHC) or Immunostaining, is a very common technique to visualize target antigens on the tissue specimen mounted on the glass slide by using antibodies. Not only general IHC, but this technique is still used in most advanced technique, such as imaging mass cytometry.

In immunohistochemistry, creating a hydrophobic barrier around tissue sections is an essential step to keep antibody solutions and reagents confined to the intended area.
Hydrophobic barrier pens are widely used for this purpose, but issues such as small gaps, insufficient drying, or accidental damage to the barrier can sometimes lead to reagent leakage during subsequent steps.
CS IHC-Fencing Seal is designed to help prevent these downstream issues by providing a pre-formed, uniform, water-repellent barrier.
Just take the seal from mounting and put it on the slide. This simple procedure will minimize all troubles caused by hydrophobic barrier pens.

In addition, when multiple tissue sections on a single slide need to be incubated with different antibodies or reagents, maintaining clear separation is particularly important. 
This physical barrier helps prevent accidental mixing during incubation and washing, supporting smoother and more reliable staining workflows.

・Stable : This barrier label is resistant to general buffers, chemicals and detergent (e.g. Triton X-100), and high temperature (70℃.)
・No leak : As there is no gap, solution will be well-retained in the circle or square.*
　* Please remove air bubbles under the frame and attach firmly.
・Time-saving : No need to wait until the line dries like hydrophobic pens. Just put it and go!
・Easy to remove : Small tab supports fast and easy removal.
・No overflow : You are free from a common trouble of hydrophobic barrier pens.
・Earth Friendly : No organic solvent is used unlike conventional hydrophobic barrier pen.
・Variety : Available in two types （Circle or Square) with different size option.

Evaluation points

1. Adherence property throughout the immunohistochemistry staining procedure
2. No outflow
3. Usability (Easy to remove and attach)

Experimental conditions

PBST: 0.1% Triton X 100 containing phosphate buffered saline
PBSMT Composition: 0.1% Triton X 100 and 2% skim milk containing phosphate buffered saline 

Evaluated products

Procedure

1. Pinch a tab and remove the CS IHC seal from mounting.

2. Put the CS IHC seal onto the glass slides.

3. Immerse the glass slides in the staining jar with 150 mL of PBSMT and incubate them at room temperature for 1 hour.

4. Remove the glass slides from the staining jar and place them in the moisture chamber.

5. Apply primary antibody solution (diluted in PBSMT) to the glass slide.

Type A-F from the top to bottom.

Summary

・Under the general IHC protocol, IHC seal completely retained throughout the procedure.
・Antibody solution tightly kept within the seal of circle/square. No outflow with recommended volume.
・The handle tab makes it easy to remove from mounting.